- Gives greater sensitivity in downstream assays
- Now includes reagents/protocol for RNA recovery from LCM samples
- Isolate high quality RNA from micro-scale samples
RNAqueous® Technology
RNAqueous kits can be used to purify total RNA from many different tissues and cells in a fast procedure without the use of phenol. Cells or tissue are disrupted in a guanidinium thiocyanate solution; this chaotropic agent effectively lyses cells and inactivates endogenous ribonucleases. The lysate is then diluted with an ethanol solution and applied to an RNA-binding glass fiber filter. Proteins, DNA, and other contaminants are removed in three rapid washing steps, and the bound RNA is then eluted in concentrated form. The entire procedure can be completed in 20 minutes after tissue disruption. No phenol:chloroform extraction, protease digestion, or alcohol precipitation steps are required, making the procedure ideal for processing many samples. The resultant RNA is suitable for use in most common applications, including Northern blots, RT-PCR, nuclease protection assays, array probe labeling, and in vitro translation. Ambion has incorporated the RNAqueous technology into a variety of products for specific applications, including micro scale RNA isolation.
RNAqueous-Micro
The RNAqueous-Micro Kit is optimized for the purification of total RNA from micro sized samples such as 10–500,000 cultured cells, as few as 10 microdissected cells, or up to 10 mg tissue. Samples are solubilized in a powerful chaotropic solution, and RNA is purified on a glass fiber filter cartridge specifically designed for very small samples. A simple modification of the protocol allows recovery of both large and small RNA species including tRNAs, 5S rRNA, and microRNAs. The RNA is eluted in a minimal volume of 20 µl. Residual genomic DNA is efficiently removed using the DNA-free system included in the kit. RNAqueous-Micro contains enough reagents for 50 RNA isolations of DNA-free RNA.
Biocompare® Product Review
Trademarks, Patents, Licensing
|
