- Highest yields of total RNA available
- No enzymatic pretreatment required
- Includes DNA-free™ Reagents
New! Isolate High Quality Total RNA from Yeast
It has traditionally been difficult to isolate high quality RNA from yeast because of problems associated with disrupting the cells. Many protocols utilize enzymatic pretreatments to disrupt the cells that may affect gene expression patterns. The RiboPure-Yeast RNA Isolation Kit uses no enzymatic pretreatments and thus does not alter expression profiles. RiboPure-Yeast combines a simple and efficient glass bead/one step phenol mediated disruption using Ambion’s Vortex Bead Beater Adapter followed by a glass fiber filter based RNA purification step. After DNase treatment with the included DNA-free™ Reagents, the resulting RNA is exceptionally pure containing little to no genomic DNA (< 0.05%). The kit has been used extensively at Ambion to isolate high quality RNA from Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris, and Candida albicans (Figure 1). The kit provides the highest yield of any method tested from each of these species (see Figure 2). The RiboPure-Yeast RNA Isolation Kit contains sufficient reagents for 50 RNA isolations each from 107 to 108 yeast cells.
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