- Efficient isolation from 100–2,000,000 cells or 0.1–1.5 mg of tissue
- High-throughput total RNA isolation
- No phenol extraction or alcohol precipitation
- Reproducible high yields
- Optional on-the-filter DNase treatment
- Also available in AUTOMATED FORMAT
Ambion's RNAqueous technology is now available in a high-throughput, 96-well plate format. The RNAqueous-96 Kit is ideal for the isolation of total RNA from multiple samples and very small samples. The procedure utilizes an RNA-binding glass-fiber filter and either vacuum filtration or centrifugation methods to provide high yields of intact RNA. An optional on-the-filter DNase treatment can be performed to ensure removal of genomic DNA for RT-PCR applications. The 96-well plates are compatible with most common 96-well plate vacuum manifolds and 96-well plate centrifuge rotors.
The RNAqueous-96 Kit provides consistently high yields of RNA from various amounts of starting material. The high quality of RNA isolated with the RNAqueous-96 Kit is illustrated by an EtBr-stained denaturing agarose gel electrophoresis (see figure at left). The RNAqueous-96 Kit includes sufficient reagents and supplies to perform 192 isolations from samples of cells or tissues.
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