- Enhanced lysis/binding conditions to accommodate larger sample inputs
- Improved TURBO DNase™ treatment to minimize DNA contamination and preserve RNA integrity
- Now includes an enhanced protocol for RNA isolation from plant tissues
- Reduced to 96 reactions per kit, more suitable for moderate throughput users
Magnetic beads offer many benefits for isolating RNA from cells and viral RNA from cell-free samples. Ambion’s MagMAX technology binds RNA more efficiently than glass fiber filter methods, resulting in higher RNA yields. Furthermore, the RNA yields are more consistent, both from experiment to experiment and over a broad range of sample sizes. Another important benefit of MagMAX over glass fiber filter-based methods is the elimination of filter clogging due to cellular particulates in samples. Since only a small volume of magnetic beads is needed for high efficiency binding, the bound RNA can be eluted in just 20–50 µl of nuclease free water. This makes the MagMAX magnetic bead technology extremely useful for concentrating RNA from large, dilute samples.
Improved! MagMAX-96 Total RNA Isolation Kit
The improved MagMAX-96 Total RNA Isolation Kit accommodates more diverse biological samples and a broader range of cell and tissue input amounts. The kit is optimized for high throughput isolation of RNA from 25 cells to 2 x 106 cells, as well as small plant and mammalian tissue samples (up to 10 mg). Ambion’s TURBO DNase™ is included to improve genomic DNA removal. The quantitative RT-PCR results shown at right demonstrate the excellent linearity obtained with this kit when total RNA was isolated from increasing numbers of K562 cells. With this kit, high quality, intact total RNA can be isolated from mammalian cells, animal tissues, and plant tissues (when combined with Ambion’s Plant RNA Isolation Aid).
The protocol is fully amenable to automation. Detailed guidelines for general automation are included with the kit, and downloadable protocols for the use of this kit with specific liquid handling systems are available at our Automation Resource.
For additional processing plates and lids, Ambion recommends Phenix #MPU-8117 (Plate) and Phenix #MPE-8019 (Lid).
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