- Compatible with both chemically and enzymatically synthesized siRNA
- Label up to 12 siRNAs, one or both strands
- Both Cy3 and Fluorescein (FAM) Labeling Kits available
The Silencer Cy3 and Fluorescein siRNA Labeling Kits are the perfect complement to the Silencer® siRNA Construction Kit and Silencer® siRNA Cocktail Kits, but can also be used to label chemically synthesized siRNAs. Two labeling kits are available: Cy3 and FAM. Each kit comes with all the reagents necessary to label 65 µg of siRNA. Labeling reactions are scaleable, but typically twelve 5 µg aliquots of siRNA are labeled per kit.
Labeled siRNA can be used to analyze siRNA subcellular localization, stability, and transfection efficiency. In addition, fluorescently labeled siRNA is particularly well-suited for use in double label experiments (with a labeled antibody) to track cells that receive siRNA during transfection and to correlate transfection with down-regulation of the target protein. Further, research indicates that labeling of siRNAs does not affect their biological function (upper figure, right).
The lower figure at right shows a silencing experiment performed with a GAPDH siRNA and corresponding scrambled siRNA sequence labeled with Cy3 using with the Silencer Cy3 siRNA Labeling Kit. The labeled siRNAs were transfected separately into HeLa S3 cells and then imaged by fluorescence microscopy. An anti-GAPDH antibody was used to monitor silencing of GAPDH expression. The siRNA to GAPDH has a strong silencing effect denoted by the lack of GAPDH protein expression (green) in Panel A compared to Panel B where the scrambled siRNA sequence was used.
Each Silencer siRNA Labeling Kit comes complete with labeling reagents for 65 µg of siRNA, a GAPDH control for labeling, and a detailed Instruction Manual.
biocompare® Product Review
Trademarks, Patents, Licensing
|
