- Broad cell line compatibility — kit includes siPORT™ Amine and siPORT™ NeoFX™ transfection agents
- Faster protocol optimization — kit includes ready-to-transfect GAPDH and negative siRNA controls
New! Silencer™ siRNA Transfection II Kit
The choice of transfection agent is critical for gene silencing experiments. Every transfection agent has a different delivery efficiency when used with different cell types, and the overall transfection efficiency and degree of gene silencing depend on the nature of the transfection agent/siRNA complex. Whether you are new to RNAi research or you want to expand your experiments to include different cell lines, Ambion's Silencer™ siRNA Transfection II Kit enables you to optimize your transfection protocols for specific cell types. For more information about how to optimize your transfection conditions, please visit our siRNA Delivery Resource.
The Silencer siRNA Transfection II Kit includes siPORT™ Amine Transfection Agent (a polyamine mixture) and the new siPORT™ NeoFX™ Transfection Agent (a cationic and neutral lipid mixture). These reagents support efficient, reproducible siRNA transfections in different cell types (Figure 1). Furthermore, siPORT Amine and siPORT NeoFX can be used to transfect cells during subculturing—saving a full day of valuable time—without increased cytotoxicity.
Controls for Optimization
In addition to containing 0.4 ml of each siPORT transfection agent, this kit includes 50 µl of a 20 µM well-characterized GAPDH synthetic siRNA that can be used to optimize transfection conditions. Results can also be verified at the protein level with GAPDH-specific antibodies (Ambion). A scrambled, negative control siRNA (30 µl of 20 µM siRNA) is provided to confirm the specificity of the siRNA of interest and to demonstrate the absence of nonspecific, toxic effects.
Two Choices = Success in More Cell Types
By offering a choice of two different transfection agents, each of which supports optimal transfection in different cell types, and by including both positive (GAPDH) and negative (scrambled) control siRNAs to facilitate transfection optimization, the Silencer siRNA Transfection II Kit will enhance successful siRNA delivery into your cells.
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