• Premium quality synthesis and purification
  
  
• You provide the sequence, we'll do the rest
  
  
• Rigorous quality control
  
  
• Your choice of purification method, amount and format
  
  
• Available annealed and ready to use
  
  

SEE RELATED PRODUCTS

Order Using GeneAssist™ siRNA Workflow Builder

Other Links

Search siRNA Database Browse Cellular Pathways for siRNAs Alternative Products: Custom Select siRNA Synthesis siRNAs synthesized with "Select" chemical modifications for enhanced specificity Silencer® Custom Designed siRNAs For needs that extend beyond our collection of pre-designed siRNAs Silencer® Pre-designed siRNAs Guaranteed-to-silence siRNAs to all human, mouse, and rat genes Silencer® Control siRNAs Labeled or unlabeled positive and negative control siRNAs

Important Licensing Information

Ambion's custom siRNA service provides the premium quality siRNAs you need for your gene silencing research. Custom siRNAs can be readily ordered online by providing either the 19–21 base target sequence, or by providing the sense and antisense siRNA sequences. (For greater convenience, consider our Silencer® Validated and Silencer® Pre-designed siRNAs, with siRNAs already designed for all human, mouse, and rat targets in the RefSeq database.) Custom synthesized siRNAs are offered in various formats and amounts, and with many different modifications, including fluorescent labels. For the enhanced specificity associated with our "select" chemical modifications, please see Custom Select siRNA Synthesis.

Premium Quality siRNA Synthesis
Each siRNA is synthesized in our state-of-the-art synthesis facility to meet the highest quality standards. Ambion's standard purification procedure, available for 21mer siRNAs, includes deprotection and column purification, and typically yields siRNA that is 90% pure (guaranteed >80% pure). For the most demanding applications, and for siRNAs of lengths other than 21 bp, HPLC purification is available, yielding siRNAs that are guaranteed >97% pure.

To ensure that the siRNAs are of highest quality, all RNA oligonucleotides are assessed by MALDI-TOF (matrix-assisted laser desorption ionization–time-of-flight) mass spectrometry, and the purity of all HPLC and PAGE purified oligonucleotides is assessed by analytical HPLC. Ambion also analyzes each annealed siRNA by gel electrophoresis to confirm that the strands annealed properly. The result is premium quality siRNA that is purified and ready to use.

In Vivo Ready siRNAs
"In Vivo Ready" custom synthesized siRNAs–the highest quality siRNAs available anywhere–are subjected to that extra level of purification and testing required for the introduction of siRNAs into animals. After HPLC purification and annealing, each siRNA is further purified using a process that removes excess salt via a semi-permeable membrane. The result is highly pure siRNA with minimal salt content, suitable for in vivo applications. In vivo siRNAs are then sterile filtered and tested for the presence of endotoxin. At a concentration of 50 µM in deionized water, in vivo Ready siRNAs contain <0.6 mM Na⁺, <2.0 mM K⁺, and <0.1 mM Mg²⁺.

<<More Information about siRNA Purity and Labeling Options

In addition to the synthesis scales and purification options described above, other scales and options are available, including 96-well synthesis. If you have a custom RNA synthesis project that falls outside the parameters above, please contact us at custom_services@ambion.com. Our custom synthesis department is ready to work with you to meet your research objectives, no matter what the scope.

Approximate Turnaround Times*
Standard purification	4 business days
HPLC purification	6 business days (20 and 40 nmol) 8 business days (160 nmol) 15 business days (250 nmol)
In Vivo Ready	12 business days (100 nmol) 15 business days (250 nmol, 1 µmol) 20 business days (10 µmol)
Cy3 and FAM labeled	8 business days

*Estimated time from order confirmation to ship date from Austin, TX for orders confirmed by 2 pm CST. Does not include time required for shipping (typically overnight for US and Canada deliveries, 2 days for Europe, 2-3 days elsewhere).

Prices quoted are for 21 bp siRNAs with dTdT or UU termini. Extra fees apply for additional bases and alternate termini. Please contact Ambion or your local distributor for additional details.

Ambion maintains strict confidentiality of customer provided information, including sequence and siRNA ordering information.

Trademarks, Patents, Licensing

RELATED PRODUCTS

Silencer® Negative Control siRNAs Silencer® Positive Control siRNAs Silencer® Pre-designed siRNAs Silencer® Validated siRNAs siPORT™ NeoFX™ Transfection Agent siPORT™ siRNA Electroporation Buffer/Kit Applied Biosystems: TaqMan® Gene Expression Assays