- Quantitative results in less than 3 hours
- Detection from as little as 25 pg of total RNA
- Suitable for either real-time or end-point RT-PCR
- Ideal for validating expression profiling data and high-throughput applications
Availability of primer sets cannot be guaranteed. Please contact customer service to confirm availability before ordering.
mirVana qRT-PCR miRNA Detection Kit
MicroRNAs (miRNAs) are evolutionarily conserved, small, non-coding RNA molecules that are estimated to regulate at least 30% of all protein encoding genes. Currently more than 2,900 miRNAs have been annotated from a wide range of organisms including plants, worms, flies, mammals, and viruses. To date, miRNAs have been implicated in development, differentiation, apoptosis, fat metabolism, viral infection, and cancer.
Due to their small size (~22 nt), miRNAs cannot be analyzed by traditional PCR approaches. Designed for use in conjunction with mirVana qRT-PCR Primer Sets, the mirVana qRT-PCR Detection Kit has been optimized to amplify specific small RNAs, enabling accurate quantitation of miRNA expression levels (Figure 2). This novel detection system can be used to characterize miRNA expression via traditional endpoint RT-PCR or real-time RT-PCR using SYBR® Green I.
Since miRNAs represent only a minute fraction of total RNA, detection of these molecules can be difficult. However, the mirVana qRT-PCR miRNA Detection Kit is extremely sensitive, detecting miRNA from as little as 25 pg of total RNA. The kit also specifically detects mature miRNAs and not miRNA precursors (Figure 3). Furthermore, the mirVana qRT-PCR miRNA Detection Kit exhibits linearity over at least 5 logs (Figure 4) and can be used with total RNA, miRNA-enriched RNA fractions, or even degraded RNA samples.
Each kit contains sufficient reagents for 200 reactions, as well as a control primer set to amplify the ubiquitously expressed miR-24 and human heart total RNA. Note: Thermostable DNA polymerase is not supplied with the kit. For analysis of individual miRNAs, the mirVana qRT-PCR miRNA Detection Kit is unparalleled in sensitivity and speed!
mirVana qRT-PCR Primer Sets
mirVana qRT-PCR Primer Sets are designed for use with the mirVana qRT-PCR miRNA Detection Kit. Each qRT-PCR Primer Set includes a primer for reverse transcription and a PCR primer pair optimized for sensitive detection of specific miRNAs by qRT-PCR.
mirVana qRT-PCR Primer Sets are available for many human, mouse, and rat miRNAs. In addition, Primer Sets specific for the small RNA species U6 snRNA and 5S rRNA are offered to control for input variability and sample normalization. See the Available Primer Sets.
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