- Fast and easy precipitation of small nucleic acids
The flashPAGE Reaction Clean-Up Kit is a fast and convenient filter-based purification/concentration system for small nucleic acids obtained using the flashPAGE™ Fractionator. During flashPAGE electrophoresis, RNA and DNA molecules are separated from longer species and collected in the lower running buffer chamber. These small nucleic acids may then be precipitated from the electrophoresis buffer using sodium acetate and ethanol. However, for quantitative recovery, the precipitation must be incubated overnight at –20°C.
The flashPAGE Reaction Clean-Up Kit is a rapid and simple alternative to overnight precipitation. Using the flashPAGE Fractionator and flashPAGE Reaction Clean-Up Kit, the entire small nucleic acid purification procedure takes less than 45 minutes, offering significant time and labor savings over traditional PAGE purification and overnight precipitation. The purified nucleic acids can be used in any downstream enzymatic or analytical applications, including array analyses, solution hybridization, and Northern blotting.
The flashPAGE Reaction Clean-Up Kit contains reagents for the purification of 20 samples obtained using the flashPAGE Fractionator. Properly stored kits are guaranteed for 6 months from the date received.
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