• Optimized for high protein yield and biological activity
  
  
• Consistent performance
  
  
• Efficiently translates a wide variety of high molecular weight proteins
  
  
• Efficiently translates both capped and uncapped mRNAs
  
  
• Accommodates large volume of template mRNA
  
  

Ambion's reticulocyte lysate efficiently translates in vitro synthesized transcripts, poly(A) and total RNA, including difficult-to-translate RNAs. The specifically formulated lysate results in high protein yield and biological activity. An optimized Translation Mix (-Met or -Leu) for the translation of both capped and uncapped messages is included along with High and Low Salt Buffers. These Buffers can be used to maximize protein yield and activity from difficult-to-translate sequences.

A report in Nucleic Acids Research (1) found that some commercial reticulocyte lysates had poor fidelity for initiation at the appropriate AUG initiator codon. Ambion's Retic Lysate IVT Kit was tested using Kozak's constructs and it demonstrated high fidelity for initiating at the proper AUG. The lysate has been optimized for translation by the addition of an ATP regenerating system, hemin, and calf liver tRNA. The lysate will efficiently translate total RNA, poly(A) RNA, and capped or uncapped in vitro transcripts. Researchers need only add labeled amino acid and RNA to the optimized lysate.

Reference
1. Kozak M (1990) Evaluation of the fidelity of initiation of translation in reticulocyte lysates from commercial sources. Nuc. Acid Res. 18: 2828.

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