UV shadowing is a quick and easy method for visualizing any nonisotopically labeled or unlabeled nucleic acid (DNA or RNA). A fluor-coated TLC plate is placed underneath the gel and a short wavelength UV light is shone down on the gel. The nucleic acid absorbs light at this wavelength and casts a shadow against the fluorescent background, allowing the full-length nucleic acid to be marked and excised from the gel. As little as 0.3 µg can be detected.

We recommend gel purification of full-length RNA transcripts away from smaller fragments for use in nuclease protection assays. UV shadowing is an ideal way to assess and gel purify nonisotopically labeled RNA probes.

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