Amino Allyl Labeling for Array Analysis
This short article discusses the differences between direct incorporation and post-synthesis reaction methods for fluorescently labeling nucleic acids for array analysis.

Array Analysis: The Basics
Learn about the different types of arrays, how they are analyzed and what steps are involved in a typical gene array experiment. This article includes a list of web resources for further reading.

Comparison of Different Methods of mRNA Quantitation
A brief comparison of nuclease protection assays, Northern blotting, RT-PCR and in situ hybridization for the detection and quantitation of RNA.

In Vitro Transcription: The Basics
This article presents an overview of in vitro transcription, including requirements of in vitro transcription reactions and a comparison of conventional vs. large scale RNA synthesis. This is a good place to start if you haven't performed in vitro transcription in the past and want to know what's involved.

In Vitro Translation: The Basics
This article explains different approaches to in vitro protein synthesis (translation of purified RNA versus "linked" and "coupled" transcription:translation) and also describes the basic differences between eukaryotic and prokaryotic cell-free systems.

Northern Analysis: The Basics
"Northern Analysis: The Basics" leads you through the steps involved in Northern blotting and explains the considerable improvements Ambion has found over standard Northern blotting protocols. If you have never performed Northern analysis, or if you want to optimize your Northern blot experiments, you will definitely want to check out this informative article.

Nuclease Protection Assays: The Basics
"Nuclease Protection Assays: The Basics" explains the advantages and limitations of nuclease protection assays (NPAs), as well as the steps involved. If you have never performed an NPA, or are confused about which NPA kit to choose, this article is for you.

Overview of RNA Interference
A comprehensive review of RNA interference (RNAi), including the mechanism of RNAi, applications of RNAi, and some guidance regarding the execution of RNAi experiments.

RNA Isolation: The Basics
Learn about the many different approaches to RNA isolation in "RNA Isolation: The Basics." This article discusses isolation procedures for both total and poly(A) RNA. It also covers the treatment and handling of tissues or cells prior to RNA isolation and storage of purified RNA, which are also critical for obtaining high-quality RNA.

RNase Control: The Basics

RT-PCR: The Basics
This article gives an overview of RT-PCR and its use for mRNA quantitation. "RT-PCR: The Basics" explains how relative and competitive RT-PCR differ, what things to watch out for, and how to choose and validate controls